Local Protocols

Here are some protocols we use in the lab.

• Extracting soluble and insoluble protein fractions
• Thoughts on lysis of yeast cells
• Resolving and detecting ubiquitin
• Using paromomycin to alter translation in yeast
• Cell preparation and protocol to view RFP/TFP fluorescence
• Using the Zeiss LSM 510 confocal microscope
• Yeast OD and cell viability model
• High Efficiency Yeast Transformation
• Yeast Genomic DNA Prep
• Isolating Total, Soluble and Insoluble Protein Fractions
• The relationship between the selection coefficient and relative growth rates: under construction

Remote Protocols

Rather rapid yeast genomic DNA prep (Gottschling Lab)
SDS-PAGE (Szostak Lab)